The absorption and emission bands of DiI-C 12 (in DMSO) are centered at 551 nm and 659 nm, respectively (Fig. Invitrogen BODIPY® FL dye is a green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Alexa Fluor 488. In the . Many fluorescent lipid probes tend to loop back to the membrane interface when attached to a lipid acyl chain rather than embedding deeply into the bilayer. . we performed not only measurements with both BODIPY-cholesterol probes in model and cell membranes but also with the B-Chol-CD and B-P-Chol-CD solutions. This . Cholesterol itself has very few structural/chemical features suitable for real-time imaging in living cells. Analysis of sterol distribution and transport in living cells has been hampered by the lack of bright, photostable fluorescent sterol derivatives that closely resemble cholesterol. Invitrogen™ CholEsteryl 4,4-Difluoro-5- (4-Methoxyphenyl)-4-Bora-3a,4a-Diaza- s -Indacene-3-Undecanoate (CholEsteryl BODIPY™ 542/563 C 11 ) Orange-red fluorescent cholesteryl ester can be used as tracer of cholesterol transport and rand as general nonexchangeable membrane marker Manufacturer: Invitrogen™ C12680 Catalog No. BODIPY-cholesterol can be reliably used to monitor cholesterol efflux ... The Hedgehog Receptor Patched Is Involved in Cholesterol Transport - PLOS Cholesteryl Ester Transfer Protein Variants Have Differential Stability ... . Invitrogen CholEsteryl 4,4-Difluoro-5-(4-Methoxyphenyl)-4 ... - Fisher Sci retinoic acid, cholesterol, actinomycin D and gemcit-abine hydrochloride (USP standard) (Sigma); insulin (ermoFisher); protein A/G agarose beads (Millipore); BODIPY dyes (ermoFisher). Bodipy-cholesterol has been shown to behave similarly to native cholesterol in cultured vertebrate cells . To achieve maximum embedding of BODIPY (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) fluorophore into the bilayer apolar region, a series of sn-2 acyl-labeled phosphatidylcholines was synthesized bearing 4,4-difluoro-1,3,5,7-tetramethyl-4 . Examination of the relationship between ApoE phenotypes and its cholesterol regulatory mechanism revealed a significantly (p<0.001) greater efflux of BODIPY-cholesterol from the non-aggressive cell lines compared to the aggressive cell lines ().The statistical summary of the reverse cholesterol transport process showed a significant .
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